UTILIZAÇÃO DE SANGUE DE ABATEDOUROS PARA PRODUÇÃO DE BACILLUS THURINGIENSIS POR FERMENTAÇÃO SUBMERSA

Abstract

Bacterial fermentation, using agroindustrial residues, was studied aiming to obtain a useful biopesticide to control insect pests of the Agriculture or Public Health. Literature deals with Biological Control and Microbial Control definitions, and this paper studied the development of Bacillus thuringiensis var. kurstaki using residues of animal blood as substrate. A rotary shaker was used and the growth conditions were: initial pH = 7.3; temperature at 30 o C; agitation rate at 150 rpm, and aeration at a 1/5 volume of the culture flask. Best results using animal blood were gotten with non-sterilized blood, being the growth over chicken blood faster than in the pork blood. The pH behavior in this case was similar to that with culture medium recognized as good sources. It was concluded that the animal blood studied was a very good source for the development of the biopesticide using Bacillus thuringiensis, besides the advantage of the reduction of this polluent material (non costly source).